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PURPOSE: Development of a hybridization technique for spermatozoa allowing the preservation of the head morphology.
METHODS: FISH analysis of fixed semen samples from oligoasthenoteratozoospermia (OAT) patients with a normal somatic karyotype attending the Cytogenetics and the IVF Laboratories of a University hospital for semen analysis. In situ hybridization with centromeric probes for chromosomes X, Y, and 18 and locus specific probe for chromosome 21.
RESULTS: More than 95% of the sperm heads showed clear hybridization signals and a conserved morphology including the visualization of the tail. Few cells with splitted signals were not considered.
CONCLUSIONS: This is the first description of a simple and fast hybridization protocol for spermatozoa without a decondensation step, allowing preservation of the morphology of the ...
AIM: To assess the prevalence of hepatitis B markers in the adult population of the Coimbra district, in the central region of Portugal. METHODS: The study included 657 subjects chosen aleatorily from 4 characteristic councils of the Coimbra district. They were questioned regarding risk factors and blood samples were taken for HBsAg, anti-HBs and anti-HBc testing. Anti-HCV was previously determined and it was positive in 0.45% of the cases. RESULTS: HBsAg was found in 0.91%. Anti-HBs and anti-HBc were both positive in 6.08%, anti-HBc without anti-HBs was found in 1.82% and anti-HBs without anti-HBc was found in 1.67% (11 cases, 8 vaccinated). The general prevalence of hepatitis B markers was 9.3%. Higher prevalences were found in the subjects over 50 years of age, 13.5% (p < 0.05), and in the urban area, 13.2% (p < 0.05). CONCLUSIONS: ...
Coventry, Maquet e outros demonstraram que as osteotomias da tíbia proximal
são uma opção no tratamento da gonartrose unicompartimental. Com o avanço no
desenho protésico e instrumentais mais precisos, a Artroplastia Total do
Joelho (ATJ) é hoje o tratamento de eleição da pangonartrose sintomática.
Contudo, o tratamento da artrose medial ou lateral combinada com artrose
patelo-femoral, em estadios iniciais e em idades jovens, mantém-se um
dilema.
Dos 24 casos avaliados, 21 (87,5%) foram osteotomias de valgização e 11
foram re-operados: 10 ATJ (tempo médio após osteotomia de 17,9 ±5,8 anos) e
1 artrodese (na sequência de uma infecção). A queixa predominante
pré-operatória foi de dor anterior do joelho com crepitação dolorosa e
sensação de instabilidade patelar (41,6%). Como complicações, 1 (4,2%) caso
de deiscência da sutu...
As cardiopatias são o grupo mais comum de anomalias congénitas.
Neste grupo, 25% são consideradas críticas e necessitam
de cirurgia ou cateterismo de intervenção durante o 1º
ano de vida. O diagnóstico atempado e precoce destas situações
melhora o prognóstico, diminuindo a morbi-mortalidade
associada. Tem havido um grande interesse na utilização da
oximetria de pulso como método de rastreio das cardiopatias
congénitas. A justificação para o seu uso reside no facto de
nalgumas cardiopatias congénitas críticas, haver algum grau
de hipoxémia mesmo antes da cianose ser clinicamente evidente.
A oximetria de pulso é um teste não invasivo, não doloroso,
fácil de executar, com grande fiabilidade na avaliação
da hipoxémia. Dada a evidência cientifica, a exequibilidade,
segurança e ganhos potenciais em saúde com a aplicação
do test...
Most authorities recommend starting biological agents upon failure of at least one disease-modifying agent in patients with rheumatoid arthritis. However, owing to the absence of head-to-head studies, there is little guidance about which biological to select. Still, the practicing clinician has to decide. This review explores the application of published evidence to practice, discussing the goals of treatment, the (in) ability to predict individual responses to therapy, and the potential value of indirect comparisons. We suggest that cycling of biological agents, until remission is achieved or until the most effective agent for that individual patient is determined, deserves consideration in the current stage of knowledge.
