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In the last years, it has been reported that bone marrow stromal cells (BMSC) are able to differentiate towards a neuronal phenotype, in vitro as well as in vivo, and consequently, the possible use of these cells for the treatment of neurological diseases has acquired enormous importance. The objective of this review is to discuss the experimental findings that suggested the utility of BMSC for the treatment of paraplegia, and the possibilities of its clinical application in patients. For this reason, we revise our previous experimental findings about neuronal transdifferentiation of BMSC, and the utility of local BMSC transplantation in an experimental model of chronic paraplegia. Our current experience supports that a neural transdifferentiation of BMSC is possible after these mesenchymal stem cells are transplanted into injured spin...
The first barometers in the Americas were provided by the Royal Society of London in 1677 to correspondents in the Caribbean Island of Barbados. Colonel William Sharpe of Barbados was the first person in the Americas to make daily observations of the weather using a meteorological instrument (other than a wind vane) and made the first known measurements of barometric pressure within the circulation of a hurricane on 12 August 1680. His record provides new insight into the early history of the barometer and early perceptions of tropical weather, vindicates the hypothesis that the barometer would prove useful in detecting hurricanes, and contributes to Edmund Halley's understanding of the empirical distinctions between the Tropics and temperate zones. Sharpe's name and contributions, previously unknown to the meteorological community, ca...
A monoclonal antibody against the surface marker IOT-10 of natural killer (NK) cells was used to investigate the presence of these cells in a series of twenty intracranial meningiomas. In al1 of these tumours, IOT- 10 positive NK cells were found in small numbers, mainly distributed among the tumor cells. Two recurrent tumors showed a relatively high number of immunostained cells. The data obtained in the present study suggest that a NKcell- mediated immunological response can occur in meningioma tissue.
A technique for staining the nucleolar organizer regions (NORs) in tumor cells applied to smears from brain tumor biopsy specimens is described. This technique provides a rapid intraoperative evaluation of the proliferative activity in cerebral neoplasms and is a valuable complement to hematoxylineosin stained smears, supporting the critera of benignity or malignancy in these tumors.
The presence of IOT-10-positive lymphocytes among the tumor-infiltrating-lymphocyte (TIL) population was studied in a series of 185 brain tumors. In most of the tumors, IOT-10-positive lymphocytes were identified, but generally they were scarce and masked among the tumor cells, suggesting that NK-cells exercise a poor participation in the tissular response against brain tumors. Isolated tumor cells showing IOT- 10-positivity were found in low-grade astrocytomas, neurinomas and medulloblastomas. IOT- 10-positivity on both tumor neuropil and tumor cells was considered a characteristic finding in oligodendrogliomas. The number of IOT- 10-positive NK-cells in brain metastases and in cerebellar hemangioblastomas was comparatively greater than in other types of brain tumor. Since in brain metastases, the presence of IOT-10-pos...
The purpose of this study is to evaluate, in an experimental model of spinal cord injury (SCI), the presence of apoptotic cell death after trauma and if early administration of a single bolus of methylprednisolone (MP) influences apoptosis in the zone of trauma and in adjacent spinal cord segments. For this study, a total of 96 adult female Wistar rats were subjected to spinal contusion at the T6-T8 level, producing immediate paraplegia. Forty-eight animals (treated group) received a single intraperitoneal injection of MP, at a dose of 30 mg/kg body weight, 10 minutes later. Cells undergoing apoptosis were detected by means of immunohistochemical labeling with the monoclonal antibody Apostain (anti-ssDNA MAb F7-26), in the injured spinal cord tissue, both in the zone of the lesion and in the adjacent spinal segments (ros...
When a severe neurological lesion occurs as a consequence of intracerebral bleeding, no effective treatment for improving the outcome is currently available. In the present study, intracerebral hemorrhage (ICH) was induced by stereotactic injection of 0.5 U of collagenase type IV in the striatum of adult Wistar rats, and three days later, intralesional administration of 2x106 allogeneic bone marrow stromal cells (BMSC) in saline (n:10), or saline only (n:10), was performed. In the following 30 days, functional outcome was evaluated in each animal by rotarod and the modified neurological severity score (mNSS) test. Progressive and functional improvement was observed in BMSC-transplanted rats compared with controls, together with morphological images suggesting that intracerebral administration of BMSC increases endogenous neurogenesis a...
We present the irnmunohistochernical study of 11 cases of intracranial cysts: two extraventricular ependymal cysts, three colloid cysts of the third ventricle, four extraventricular choroidal cysts and two Rathke's cleft cysts. Antibodies against glial fibrillary acidic protein (GFAP), cytokeratins (AE1, CKSD, AE3), S-100 protein, epithelial membrane antigen (EMA), vimentin, neuron specific enolase (NSE), neurofilaments protein (NF) and prealbumin, were used. The epithelium of choroidal cysts, showed strong immunoreactivity for Prealbumin and cytokeratins, similar to the normal choroid plexus epithelium. The ependymal cysts showed epithelial immunoreactivity for GFAP and S-100, both glial markers expressed by the normal ependymal epithelium. On the contrary, the epithelial wall of colloid cysts and Rathke's cleft cyst, e...
This paper documents a rare spell of severe weather in Spain that took place during the mid-nineteenth century when a tropical storm struck the southwest of the country on 29 October 1842. The use of a variety of independent documentary sources has provided unprecedented scope for the analysis of this event, allowing it to be set within its wider context, and for a judgement to be made on its tropical origin. The evidence suggests that this was similar, though stronger, to the more recent Hurricane Vince, which made landfall in Spain on 10 October 2005. This case study not only places Hurricane Vince, suggested at the time to have been unique, in its more proper long-term context, but it also demonstrates how documentary sources can improve our wider understanding of climate dynamics during historical times in the Atlantic basin.